Publication
Molecular epidemiology of HIV type 1 infection in Portugal: high prevalence of non-B subtypes
| dc.contributor.author | Esteves, A | |
| dc.contributor.author | Parreira, R | |
| dc.contributor.author | Venenno, T | |
| dc.contributor.author | Franco, M | |
| dc.contributor.author | Piedade, J | |
| dc.contributor.author | Sousa, JG | |
| dc.contributor.author | Canas-Ferreira, W | |
| dc.date.accessioned | 2013-03-12T14:48:43Z | |
| dc.date.available | 2013-03-12T14:48:43Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | In this study, we have investigated the diversity of current HIV-1 strains circulating in the metropolitan area of Lisbon, Portugal. A total of 217 HIV-1-positive blood samples, collected between October 1998 and December 2000, was genetically characterized in the gp120 C2V3C3 region (n = 205) or part of the gp41 N-terminal segment (n = 12) by heteroduplex mobility assay (HMA) and/or DNA sequencing. The HMA subtyping efficiency (number of samples unambiguously subtyped by HMA divided by the total number of samples subtyped) was 65.9% (143 of 217), with indeterminate migration patterns of subtype A and G strains contributing significantly to this value. On the overall, subtype B was the most prevalent (50.2%), followed by subtypes G (21.7%), A (17.5%), and F (5.5%), whereas subtypes C, D, H, and J accounted altogether for 5.1% of the infections. Non-B subtypes were responsible for 77.4 and 33.1% of the infections among African immigrants and Portuguese subjects, respectively. Angolan individuals (n = 25) were the only ones infected with all the HIV-1 subtypes documented, probably reflecting a high degree of viral genetic diversification in their country of origin. Phylogenetic analysis showed a predominance of IbNG-like viruses among subtype A sequences and two new major subclusters within subtype G (G(P) and G(P)'). The majority of the Portuguese G sequences described formed a well-defined subcluster (G(P)), supported by bootstrap values >90%, phylogenetically distant from clade G sequences in databases. gag (p24/p7) sequence analysis of these variants confirmed the maintenance of the subtype G subclusters. The multiple subclustering observed for the major clades A, B, D, and G, as well as the variety of subtypes found, indicate a high diversity of HIV-1 variants circulating in Portugal and suggest a need for continuous epidemiologic surveillance. | por |
| dc.identifier.citation | AIDS Res Hum Retroviruses. 2002 Mar 20;18(5):313-25 | por |
| dc.identifier.issn | 1931-8405 | |
| dc.identifier.uri | http://hdl.handle.net/10400.10/882 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Mary Ann Liebert | por |
| dc.subject | HIV infections | por |
| dc.subject | Epidemiology | por |
| dc.subject | Portugal | por |
| dc.subject | Infecção por HIV | por |
| dc.title | Molecular epidemiology of HIV type 1 infection in Portugal: high prevalence of non-B subtypes | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Larchmont, NY | por |
| oaire.citation.endPage | 325 | por |
| oaire.citation.startPage | 313 | por |
| oaire.citation.title | AIDS Research and Human Retroviruses. | por |
| oaire.citation.volume | 18 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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