Publication
ELISA methods comparison for the detection of auto-antibodies against apolipoprotein A1
| dc.contributor.author | Frias, MA | |
| dc.contributor.author | Virzi, J | |
| dc.contributor.author | Batuca, J | |
| dc.contributor.author | Alves, JD, et al. | |
| dc.date.accessioned | 2019-05-07T14:06:07Z | |
| dc.date.available | 2019-05-07T14:06:07Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | BACKGROUND: Autoantibodies against apolipoprotein A1 (anti-apoA1 IgG) have emerged as an independent biomarker for cardiovascular disease and mortality. Across studies, different ELISA methods have been used to measure the level of circulating anti-apoA1 IgG which could lead to substantial result differences between assays. OBJECTIVES: To make a comparative study of available anti-apoA1 IgG detection methods and to determine whether the choice of matrix sample (serum vs plasma) could influence the results. METHODS: Blood samples were obtained from 160 healthy blood donors and collected on 4 different matrixes (serum, plasma-EDTA, -citrate, -lithium-heparinate). Anti-apoA1 IgG was measured using two homemade (Geneva's and Lisbon's) and one commercial ELISA kits. Passing-Bablok and Bland-Altman were used to compare the results. Anti-apoA1 IgG seropositivity cut-offs were defined according to the user's/manufacturer's criterion. RESULTS: The current results showed substantial differences between those 3 assays. The dynamic ranges were significantly different, the commercial kit displaying the narrowest one. Passing-Bablok analysis demonstrated important proportional and constant biases between assays. The anti-apoA1 IgG seropositivity rate in Geneva, Lisbon and commercial assays varied between 24.5% and 1.9%. Matrix comparisons demonstrated that the matrix choice (plasma versus serum) influenced anti-apoA1 IgG results as well as the seropositivity rate in an assay-dependent manner. The coating antigen source was identified as important factor underlying results heterogeneity across assays. CONCLUSIONS: These results highlight the impact of the method and the cut-off used on anti-apoA1 IgG results and emphasize the need of standardizing existing assays. Given the important matrix influence, we suggest to use serum as matrix of choice. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | J Immunol Methods. 2019 Jun;469:33-41 | pt_PT |
| dc.identifier.doi | 10.1016/j.jim.2019.03.011 | pt_PT |
| dc.identifier.issn | 1872-7905 | |
| dc.identifier.uri | http://hdl.handle.net/10400.10/2230 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | file://u_share/users/arminda.m.sustelo/Downloads/1-s2.0-S0022175919300407-main.pdf | pt_PT |
| dc.subject | Biomarkers | pt_PT |
| dc.subject | Cardiovascular diseases | pt_PT |
| dc.subject | ELISA | pt_PT |
| dc.title | ELISA methods comparison for the detection of auto-antibodies against apolipoprotein A1 | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Amsterdam | pt_PT |
| oaire.citation.title | Journal of Immunological Methods | pt_PT |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |