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Mutational mechanism for DAB1 (ATTTC)n insertion in SCA37: ATTTT repeat lengthening and nucleotide substitution

2019Journal article Restricted access
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dc.contributor.authorLoureiro, J
dc.contributor.authorOliveira, C
dc.contributor.authorMota, C
dc.contributor.authorCastro, A
dc.contributor.authorCosta, C
dc.contributor.authorLoureiro, J, et al.
dc.date.accessioned2020-01-31T14:51:13Z
dc.date.available2020-01-31T14:51:13Z
dc.date.issued2019
dc.description.abstractDynamic mutations by microsatellite instability are the molecular basis of a growing number of neuromuscular and neurodegenerative diseases. Repetitive stretches in the human genome may drive pathogenicity, either by expansion above a given threshold, or by insertion of abnormal tracts in nonpathogenic polymorphic repetitive regions, as is the case in spinocerebellar ataxia type 37 (SCA37). We have recently established that this neurodegenerative disease is caused by an (ATTTC)n insertion within an (ATTTT)n in a noncoding region of DAB1. We now investigated the mutational mechanism that originated the (ATTTC)n insertion within an ancestral (ATTTT)n . Approximately 3% of nonpathogenic (ATTTT)n alleles are interspersed by AT-rich motifs, contrarily to mutant alleles that are composed of pure (ATTTT)n and (ATTTC)n stretches. Haplotype studies in unaffected chromosomes suggested that the primary mutational mechanism, leading to the (ATTTC)n insertion, was likely one or more T>C substitutions in an (ATTTT)n pure allele of approximately 200 repeats. Then, the (ATTTC)n expanded in size, originating a deleterious allele in DAB1 that leads to SCA37. This is likely the mutational mechanism in three similar (TTTCA)n insertions responsible for familial myoclonic epilepsy. Because (ATTTT)n tracts are frequent in the human genome, many loci could be at risk for this mutational process.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHum Mutat. 2019 Apr;40(4):404-412pt_PT
dc.identifier.doi10.1002/humu.23704pt_PT
dc.identifier.issn1098-1004
dc.identifier.urihttp://hdl.handle.net/10400.10/2390
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/humu.23704pt_PT
dc.subjectSpinocerebellar ataxia type 37pt_PT
dc.titleMutational mechanism for DAB1 (ATTTC)n insertion in SCA37: ATTTT repeat lengthening and nucleotide substitutionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceNew Yorkpt_PT
oaire.citation.titleHuman mutationpt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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