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Polymicrobial interactions influence the agr copy number in Staphylococcus aureus isolates from diabetic foot ulcers

2018Journal article Restricted access
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dc.contributor.authorMatias, C
dc.contributor.authorSerrano, I
dc.contributor.authorVan-Harten, S
dc.contributor.authorMottola, C
dc.contributor.authorMendes, J
dc.contributor.authorTavares, L
dc.contributor.authorOliveira, M
dc.date.accessioned2019-01-28T15:39:32Z
dc.date.available2019-01-28T15:39:32Z
dc.date.issued2018
dc.description.abstractDiabetic foot ulcers are a major complication of diabetes and are often colonised by complex bacterial communities, where Staphylococcus aureus is frequently co-present with Pseudomonas aeruginosa. These bacteria interact through quorum sensing, encoded in S. aureus by the accessory gene regulator (agr). Typing and copy number of S. aureus agr were assessed here to give insights on strain variability and possible interspecies influence. As agr is classified in four genetic groups, agr-I, agr-II, agr-III and agr-IV, the agr type of 23 S. aureus diabetic foot ulcers isolates was evaluated by PCR and gene copy number determined by qPCR, including in S. aureus present in polymicrobial infections. agr-I and agr-II were found to be present in 52 and 39% of the isolates, respectively. In two isolates, no agr type was identified, and types III and IV were not detected. Interestingly, agr-II copy number was higher in dual suspensions than in S. aureus single suspension. We conclude that agr type I was the most frequent in clinical centers in Lisbon, and variations in agr-I and agr-II copy numbers were strain specific. Variations in agr copy number in dual suspensions suggests that P. aeruginosa may influence S. aureus agr-II gene regulation, confirming an interaction between these two bacteria. This is a first approach to characterise agr variation in S. aureus from diabetic foot ulcers in vitro.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntonie Van Leeuwenhoek. 2018 Nov;111(11):2225-2232.pt_PT
dc.identifier.doi10.1007/s10482-018-1103-zpt_PT
dc.identifier.issn1572-9699
dc.identifier.urihttp://hdl.handle.net/10400.10/2095
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectDiabetic footpt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectStaphylococcal infectionspt_PT
dc.titlePolymicrobial interactions influence the agr copy number in Staphylococcus aureus isolates from diabetic foot ulcerspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceBerlinpt_PT
oaire.citation.endPage2232pt_PT
oaire.citation.startPage2225pt_PT
oaire.citation.titleAntonie van Leeuwenhoekpt_PT
oaire.citation.volume111pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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