Browsing by Author "Sutre, A"
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- Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.Publication . David, S; Sutre, A; Sanca, A; Mané, A; Henriques, V; Portugal, C; Sancho, L; Cardoso, A; Paixão, E; Duarte, E; Leite, C; Salem, J; Antunes, AThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.