Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.

Palavras-chave

Tuberculose Tuberculosis Clinical laboratory techniques Mycobacterium tuberculosis

URI

http://hdl.handle.net/10400.10/990

Citação

Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7

Editora

International Union against Tuberculosis and Lung Disease

Coleções

PATCLIN - Artigos publicados em revistas não indexadas

Ver registo completo