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Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.

2012Journal article Restricted access
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dc.contributor.authorDavid, S
dc.contributor.authorSutre, A
dc.contributor.authorSanca, A
dc.contributor.authorMané, A
dc.contributor.authorHenriques, V
dc.contributor.authorPortugal, C
dc.contributor.authorSancho, L
dc.contributor.authorCardoso, A
dc.contributor.authorPaixão, E
dc.contributor.authorDuarte, E
dc.contributor.authorLeite, C
dc.contributor.authorSalem, J
dc.contributor.authorAntunes, A
dc.date.accessioned2013-08-12T16:52:13Z
dc.date.available2013-08-12T16:52:13Z
dc.date.issued2012
dc.description.abstractThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.por
dc.identifier.citationInt J Tuberc Lung Dis. 2012 Nov;16(11):1535-7por
dc.identifier.issn1027-3719
dc.identifier.urihttp://hdl.handle.net/10400.10/990
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInternational Union against Tuberculosis and Lung Diseasepor
dc.subjectTuberculosepor
dc.subjectTuberculosispor
dc.subjectClinical laboratory techniquespor
dc.subjectMycobacterium tuberculosispor
dc.titleTuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceParispor
oaire.citation.endPage1537por
oaire.citation.startPage1535por
oaire.citation.titleThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.por
oaire.citation.volume16por
rcaap.rightsclosedAccesspor
rcaap.typearticlepor

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